Table 2 List of antibodies used for immunohistochemical studies.
Antibody | Pre-Treatment | Dilution | Incubation | Cat No. | Source | Control Tissue |
|---|---|---|---|---|---|---|
Glutathione S-Transferase pi GST-Ï€ (polyclonal) | None | 1:150 | 30 min RT | PU249-UP | BioGenex (Distributor: Menarini Diagnostics, Wokingham, UK) | Breast Ca |
P-glycoprotein (MDR-1) (Clone JSB-1) | Pressure Cook 2 min pH 6.0 | 1:100 | Overnight 4°C | NCL-JSB1 | Novo Castra Newcastle-upon-Tyne, UK | Kidney |
Multidrug Resistant-Related Protein (MRP) (Clone MRPm6, specific for MRP-1) | Pressure Cook 2 min pH 7.0 | 1:30 | 30 min RT | MAB4122 | Chemicon International Chandlers Ford, UK | Kidney |
Thymidylate Synthase TS (Clone TS 106) | Pressure Cook 2 min pH 6.0 | 1:20 | 30 min RT | MS471P | Neo Markers (Distributor: Lab Vision, Newmarket, UK) | Colon Ca |