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Figure 1 | BMC Cancer

Figure 1

From: MDM2 negatively regulates the human telomerase RNA gene promoter

Figure 1

mdm2 interacts with the hTR core promoter in vivo. Formaldehyde cross-linked chromatin was prepared from 5637 cells and immunoprecipitated with antibodies to Sp1 (lane 4), TFIIB (lane 5) and mdm2 (lane 6), or in the absence of antibody (lane 3). PCR detection of DNA sequences immunoprecipitated with each antibody is shown in these lanes. PCR was performed with specific primers for the hTR promoter and for the GAPDH coding region as a negative control. A sample representative of the total input chromatin (input DNA lanes 1 and 2) was included in the PCR analysis. Lanes 7 and 8 show PCR positive and negative controls. Chromatin immunoprecipitation experiments were repeated three times and PCR analysis of individual experiments was also performed at least twice.

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