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Table 2 Mutation analysis in 20 samples by SSCP and direct sequencing

From: Prevalence of von Hippel-Lindau gene mutations in sporadic renal cell carcinoma: results from the Netherlands cohort study

  

Direct sequencing

  

Positive

Negative

Primerset 3/4

SSCP

Positive

0

5

 

Negative

0

15

Primerset 5/6

SSCP

Positive

2

0

 

Negative

0

18

Primerset 7/8

SSCP

Positive

4

2

 

Negative

0

14

Primerset 9/10

SSCP

Positive

3

0

 

Negative

0

17

Primerset 11/12

SSCP

Positive

4

1

 

Negative

0

15

Primerset 13/14

SSCP

Positive

2

0

 

Negative

0

18

  1. Positive on SSCP means: indication of a mutation or not assessable after two attempts.
  2. Positive on direct sequencing means: proof of a mutation.
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BMC Cancer

ISSN: 1471-2407

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