Figure 10

Knocking down TRAIL-R4 expression sensitized MCF7 breast cancer cells to TRAIL. A DcR2 siRNA approach was administered as described in Methods using TRAIL resistant MCF7 breast cancer cell line. Panel A depicts a flow cytometry analysis confirming strong attenuation of TRAIL-R4 expression on cell surface. TRAIL-R2 death receptor expression was also detected as a control. Sensitization of MCF7 breast cancer cells to TRAIL following a DcR2 siRNA approach is provided in Panel B. MCF7 breast cancer cells were infected with increasing doses of Ad5hTRAIL alone following a DcR2 siRNA transfection. Cell death was detected 48 hours following the infection (Panel B). Data represent the mean (± SEM) of 6 independent data points.