Figure 1
From: Maspin overexpression modulates tumor cell apoptosis through the regulation of Bcl-2 family proteins
Maspin expression in TM40D stable cell lines. Cells were stained with an AbS4A maspin primary antibody and followed by Texas-red conjugated secondary antibody. Immunofluorescence staining was carried out using TC (A, C), T16 (B, E), and T18 cells (C, F). G. High magnification of immunofluorescence staining of T18 (100× oil len) under non-permeabilized condition. Arrows indicate cell surface staining of maspin. Nuclei were stained with DAPI (D, E, F, and G). H. Quantitation of maspin expression in TC (lane 1), T16 (lane 2), and T18 (lane 3) by RT-PCR and Western blot analysis. L19 serves as a loading control for RT-PCR analysis.