Irradiation specifically sensitises solid tumour cell lines to TRAIL mediated apoptosis

BMC Cancer

Table 1 Normal tissue toxicity.

	Hepatocytes	HMEC	PrEC	SAEC	HUVEC	SMC
Control	0,3 ± 0,5	0,3 ± 0,5	0,3 ± 0,5	0,3 ± 0,5	0,2 ± 0,2	0,5 ± 0,2
TRAIL 1,0 ng/ml	0,3 ± 0,5	0,5 ± 0,4	0	0	0,2 ± 0,1	0,1 ± 0,1
10 Gy	0	0,5 ± 0,5	0	0,1 ± 0,2	0,3 ± 0,2	1,5 ± 0,5
Simultaneous combined therapy	0	1,6 ± 0,5	0,7 ± 0,5	0	0,3 ± 0,1	1,3 ± 0,9
Sequential combined Therapy	0,3 ± 0,5	0,8 ± 0,5	1,5 ± 1,0	0,3 ± 0,5	0,2 ± 0,2	1,2 ± 1,0

Cells were treated with 1,0 ng/ml TRAIL, 10 Gy, simultaneous combined therapy and with 10 Gy 12 h prior to TRAIL application. Microscopic evaluation of Hoechst stained cell nuclei with strict apoptotic criteria as chromatin condensation and nuclear fragmentation was performed 48 hours after treatment. Table shows percentage of apoptotic cells ± SD (n = 3).

ISSN: 1471-2407