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Table 1 Expression of EBAG9 and 22-1-1 on cancer cell lines.

From: Reevaluation of the 22-1-1 antibody and its putative antigen, EBAG9/RCAS1, as a tumor marker

Cell line

Origin

22-1-1 expression Flow cytometry

EBAG9 expression Immunoblot

MCF-7

breast carcinoma

++

+++

K562

chronic myelogenous leukemia

-

+++

Jurkat

T cell leukemia

+++

+++

Colo-205

colorectal carcinoma

-

+++

SiSo

cervix carcinoma

+

+++

CAL-51

breast carcinoma

-

+++

T-47D

breast carcinoma

-

+++

ZR-75-1

breast carcinoma

-

+++

HBL-100

breast carcinoma

-

+++

U-266

multiple myeloma

-

++

CaCo-2

colon carcinoma

-

++

MDA-MB-461

breast carcinoma

-

+

HEK293 A

embryonal kidney

-

+

U-373

glioblastoma-astrocytoma

-

+

L-1236

Hodgkin's lymphoma

-

+

H-184 A1

normal breast

-

+

SW-480

colon carcinoma

-

+

MDA-MB-468

breast carcinoma

-

+

  1. Expression of antigen on the cell surface was determined by incubation with 22-1-1 antibody and analysed by flow cytometry. Expression of EBAG9 was determined by immunoblot using the anti-EBAG9 serum. (+++, very high expression, ++, high expression, +, intermediate expression, -, no expression).