Figure 4

EBAG9 is sensitive to treatment with BFA and nocodazole. Displacement of EBAG9 from Golgi structures upon treatment with BFA. Cells stably transfected with EBAG9-GFP were left untreated (w/o) (a, b, c), incubated for 15 min (d, e, f) and 60 min (g, h, i) with BFA (5 μg/ml) or with nocodazole -Noc (10 μg/ml) (m, n, o) for 2 h. Cells were fixed and stained with anti-mannosidase and anti-TGN38 antibodies (red). Merged image, yellow. Bar; 10 μm