Adhesion capacity of HT-29, DanG, Caki I, and DU-145 tumor cells. Each tumor cell type was pretreated with 0.1 or 1 μM MMF or remained untreated (control) and was then added for 60 min to human umbilical vein endothelial cell monolayers. Non-adherent tumor cells were washed off in each sample, the remaining cells were fixed and counted in five different fields (5 × 0.25 mm2) using a phase contrast microscope. Adhesion capacity is depicted as tumor cell binding and related to 100% binding of non-treated control cell lines (mean ± SD; n = 6). Asterix indicates statistically significant difference to the control.