Figure 2From: Acquisition of anoikis resistance in human osteosarcoma cells does not alter sensitivity to chemotherapeutic agents Apoptosis can be induced in SAOSar cells while cultured under suspended conditions. Apoptosis was triggered in suspended anoikis-resistant SAOSar cells by staurosporine-induced inhibition of calcium-dependent protein kinases, by cycloheximide-induced inhibition of protein synthesis and by H2O2-induced oxidative damage. Percentage apoptosis was determined by flow cytometry analyzes after staining with propidium iodide. The percentage of cells in the sub-G/0 phase representative of apoptotic cells is marked on each histogram. Results shown are representative of three independent experiments.Back to article page