Apoptosis can be induced in SAOSar cells while cultured under suspended conditions. Apoptosis was triggered in suspended anoikis-resistant SAOSar cells by staurosporine-induced inhibition of calcium-dependent protein kinases, by cycloheximide-induced inhibition of protein synthesis and by H2O2-induced oxidative damage. Percentage apoptosis was determined by flow cytometry analyzes after staining with propidium iodide. The percentage of cells in the sub-G/0 phase representative of apoptotic cells is marked on each histogram. Results shown are representative of three independent experiments.