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Figure 2 | BMC Cancer

Figure 2

From: Acquisition of anoikis resistance in human osteosarcoma cells does not alter sensitivity to chemotherapeutic agents

Figure 2

Apoptosis can be induced in SAOSar cells while cultured under suspended conditions. Apoptosis was triggered in suspended anoikis-resistant SAOSar cells by staurosporine-induced inhibition of calcium-dependent protein kinases, by cycloheximide-induced inhibition of protein synthesis and by H2O2-induced oxidative damage. Percentage apoptosis was determined by flow cytometry analyzes after staining with propidium iodide. The percentage of cells in the sub-G/0 phase representative of apoptotic cells is marked on each histogram. Results shown are representative of three independent experiments.

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