Table 2 Oligonucleotide primer sequences
TP53, exons 2 to 11 (GeneBank Accession: X54156) | |||
|---|---|---|---|
Exon | Primer | Sequence 5'→3' | Fragment (bp) |
PU2 (sense) | TCCTCTTGCAGCAGCCAGACTGC | ||
PD3 (antisense) | AACCCTTGTCCTTACCAGAACGTTG | ||
4 | PU4 (sense) | CACCCATCTACAGTCCCCCTTGC | 307 |
PD4 (antisense) | CTCAGGGCAACTGACCGTGCAAG | ||
5 | PU5 (sense) | CTCTTCCTACAGTACTCCCCTGC | 211 |
PD5 (antisense) | GCCCCAGCTGCTCACCATCGCTA | ||
6 | PU6 (sense) | GATTGCTCTTAGGTCTGGCCCCTC | 182 |
PD6 (antisense) | GGCCACTGACAACCACCCTTAACC | ||
7 | PU7 (sense) | GTGTTATCTCCTAGGTTGGCTCTG | 139 |
PD7(antisense) | CAAGTGGCTCCTGACCTGGAGTC | ||
8 | PU8 (sense) | ACCTGATTTCCTTACTGCCTCTTGC | 200 |
PD8 (antisense) | GTCCTGCTTGCTTACCTCGCTTAC | ||
9 | PU9 (sense) | GCCTCTTTCCTAGCACTGCCCAAC | 102 |
PD9 (antisense) | CCCAAGACTTAGTACCTGAAGGGTG | ||
10 | PU10 (sense) | TGTTGCTGCAGATCCGTGGGCGT | 130 |
PD10 (antisense) | GAGGTCACTCACCTGGAGTGAGC | ||
11 | PU11 (sense) | TGTGATGTCATCTCTCCTCCCTGC | 153 |
PD11 (antisense) | GGCTGTCAGTGGGGAACAAGAAGT | ||
CDKN1A, exon 2 (GeneBank Accession: AF497972) | |||
p21-L1 (sense) | GATGTCCGTCAGAACCCATG | 258 | |
p21-R1 (antisense) | TGCCTCCTCCCAACTCAT | ||
p21-L2 (sense) | ATGAGTTGGGAGGAGGCA | 181 | |
p21-R2 (antisense) | ATGCTGGTCTGCCGCCGTT | ||
CDKN2A, exons 1(GeneBank Accession: U12818) and 2 (GeneBank Accession: U12819) | |||
1 | MTS1-L1 (sense) | GAAGAAAGAGGAGGGGCTG | 340 |
MTS1R1 (antisense) | GCGCTACCTGATTCCAATTC | ||
2 | p16-L2 (sense) | GTCATGATGATGGGCAGC | 307 |
p16-R2 (antisense) | CTGAGGGACCTTCCGCG | ||
CDKN2B, exon 2 (GeneBank Accession: AF513858) | |||
MTS2-L2 (sense) | TAAGTTTAACCTGAAGGTGG | 500 | |
MTS2-R (antisense) | GGGTGGGAAATTGGGTAAG | ||