New single nucleotide polymorphism found in intron 1 of the TP53 gene (C>T transition) at position 11701. (A) PCR-SSCP analysis of melanoma tumors. The arrow indicates the shifted band in melanoma tumor M71. DC, denaturing control; NDC, non-denaturing control. (B) DNA sequence of melanoma tumor M71 and autologous PBLs showing the C to T base change (shown by an asterisk *) at position 11701 compared with control PBLs.