Figure 5From: Receptor and secreted targets of Wnt-1/β-catenin signalling in mouse mammary epithelial cellsHig2-EGFP fusion protein is secreted from transfected cells. Western blot analysis of total cell lysates and conditioned medium from (1) untransfected HEK293 cells and cells transfected with plasmids encoding (2) EGFP, (3) Hig2-EGFP, (4) Hig2(Y8V9/D8D9)EGFP. Oligomers of HIG2-EGFP formed by intermolecular disulphide bridges are indicated by arrowheads.Back to article page