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Table 2 3H-proline incorporation into collagen fibres

From: Human desmoid fibroblasts: matrix metalloproteinases, their inhibitors and modulation by Toremifene

 

Cells

Media

 

3 h

Normal fibroblasts

1,060 ± 85

998 ± 94

Desmoid fibroblasts

885 ± 60

991 ± 39

Desmoid fibroblasts + toremifene

1,112 ± 134

837 ± 36

 

24 h

Normal fibroblasts

2,313 ± 99

6,523 ± 388

Desmoid fibroblasts

3,340 ± 205*

11,641 ± 421*

Desmoid fibroblasts + toremifene

2,286 ± 124°°

9,962 ± 644°°

 

48 h

Normal fibroblasts

4,262 ± 280

17,955 ± 1,073

Desmoid fibroblasts

5,649 ± 103*

32,988 ± 1,321*

Desmoid fibroblasts + toremifene

3,295 ± 261°

20,407 ± 980°

  1. Fibroblasts were cultured for 3, 24 and 48 h in MEM containing 3H-proline with or without toremifene. Total radioactivity value is expressed in cpm/mg protein and represents the mean ± SD of three determinations, each in quadruplicate. The statistical analysis was performed with analysis of variance (ANOVA). Differences vs normal fibroblasts: F-test significant at *99%; differences vs desmoid fibroblasts: F-test significant at °99%, °°95%.