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Table 2 3H-proline incorporation into collagen fibres

From: Human desmoid fibroblasts: matrix metalloproteinases, their inhibitors and modulation by Toremifene

  Cells Media
  3 h
Normal fibroblasts 1,060 ± 85 998 ± 94
Desmoid fibroblasts 885 ± 60 991 ± 39
Desmoid fibroblasts + toremifene 1,112 ± 134 837 ± 36
  24 h
Normal fibroblasts 2,313 ± 99 6,523 ± 388
Desmoid fibroblasts 3,340 ± 205* 11,641 ± 421*
Desmoid fibroblasts + toremifene 2,286 ± 124°° 9,962 ± 644°°
  48 h
Normal fibroblasts 4,262 ± 280 17,955 ± 1,073
Desmoid fibroblasts 5,649 ± 103* 32,988 ± 1,321*
Desmoid fibroblasts + toremifene 3,295 ± 261° 20,407 ± 980°
  1. Fibroblasts were cultured for 3, 24 and 48 h in MEM containing 3H-proline with or without toremifene. Total radioactivity value is expressed in cpm/mg protein and represents the mean ± SD of three determinations, each in quadruplicate. The statistical analysis was performed with analysis of variance (ANOVA). Differences vs normal fibroblasts: F-test significant at *99%; differences vs desmoid fibroblasts: F-test significant at °99%, °°95%.