Western blot analysis of MMP-1, MMP-2, MMP-9 secreted into the medium. Panel A: MMP-1; panel B: MMP-2; panel C: MMP-9. The samples are as follows: NF, normal fibroblasts; DF, desmoid fibroblasts; DFT, desmoid fibroblasts plus toremifene. Similar results were obtained in four separate experiments. Panel D. The absolute counts, obtained by densitometric analysis, were converted to percentages assuming the level of normal fibroblasts as 100%. ND = not determined.