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Figure 4 | BMC Cancer

Figure 4

From: Human desmoid fibroblasts: matrix metalloproteinases, their inhibitors and modulation by Toremifene

Figure 4

Zymogram of media from normal fibroblasts, desmoid fibroblasts, desmoid fibroblasts plus toremifene. Collagen zymogram. One set of samples was treated with APMA to activate the proenzymatic forms. Panel A. NF: normal fibroblasts, DF: desmoid fibroblasts, DFT: desmoid fibroblasts plus toremifene. In the same zymogram an aliquot of samples was activated with APMA: NF: normal fibroblasts, DF: desmoid fibroblasts, DFT: desmoid fibroblasts plus toremifene, C: trypsin. Similar results were obtained in four separate experiments. Panel B. The absolute counts, obtained by densitometric analysis, were converted to percentages assuming the level of normal fibroblasts as 100%. Gelatin zymogram. One set of samples was treated with APMA to activate the proenzymatic forms. Panel C. NF: normal fibroblasts, DF: desmoid fibroblasts, DFT: desmoid fibroblasts plus toremifene. In the same zymogram an aliquot of samples was activated with APMA: NF: normal fibroblasts, DF: desmoid fibroblasts, DFT: desmoid fibroblasts plus toremifene. Similar results were obtained in four separate experiments. Panel D. The absolute counts, obtained by densitometric analysis, were converted to percentages assuming the level of normal fibroblasts as 100%.

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