Figure 4From: Evaluation of dendritic cells loaded with apoptotic cancer cells or expressing tumour mRNA as potential cancer vaccines against leukemiamRNA transfectation of DCs. (a) Flow cytometric analysis of DCs after transfection with EGFP/pCIpA102 mRNA (10 μg/400 μl) by square-wave electroporation and maturation for 24 h in medium with maturation cocktail. Control cells were mock electroporated without mRNA. (b) Fluorescence microscopy of DCs 24 h after transfection with EGFP/pCIpA102 mRNA.Back to article page