Figure 7
A Localization of mouse S100A7/psoriasin expression to areas of squamous differentiation in skin Sections from formalin fixed paraffin-embedded normal mouse skin samples matched to the frozen tissue from which RNA was previously isolated for RT-PCR analysis were used for in situ hybridization analysis of mouse S100A7/psoriasin expression. A, B, C increasing magnifications (original is 200X) of sections hybridized with mouse S100A7/psoriasin 35S-labeled antisense cRNA, showing strong focal expression of mouse S100A7/psoriasin RNA in a particular subset of cells surrounding the hair shafts, which are associated with squamous differentiation. D, adjacent section hybridized with mouse S100A7/psoriasin 35S-labeled sense cRNA. B Upregulation of mouse S100A7/psoriasin expression in a model of skin inflammation Sections from formalin fixed paraffin-embedded mouse skin tissue at 0 mins and 24 hours after croton application to the tail skin as described in Materials and Methods. A and D, haematoxylin and eosin stained sections, from 0 and 24 hr treated skin, respectively. B and E adjacent sections from 0 and 24 hr croton oil treated skin, respectively, hybridized with mouse S100A7/psoriasin 35S-labeled sense cRNA. C and F, adjacent sections from 0 and 24 hr croton oil treated skin, respectively, hybridized with mouse S100A7/psoriasin 35S-labeled antisense cRNA.