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Figure 2 | BMC Cancer

Figure 2

From: Expression analysis of the mouse S100A7/psoriasin gene in skin inflammation and mammary tumorigenesis

Figure 2

A Top panel Expression of mouse S100A7/psoriasin in normal skin RNA from normal mouse skin from 6 different animals (1–6) was subjected to RT-PCR analysis using mouse psoriasin-ORF primers and β-actin primers as described in Materials and Methods. PCR products of 344 bp for mouse S100A7/psoriasin and 330 bp for β-actin are shown. Bottom panel Expression of mouse S100A7/psoriasin in normal mouse mammary gland) and DMB A induced mammary tumors RNA from freshly frozen matched normal mammary gland (Normal MG) and DMBA induced mammary tumors (MG tumor) from 6 different CD1 mice (1–6) was extracted and subjected to RT-PCR analysis using mouse psoriasin-ORF primers and β-actin primers as described in Materials and Methods. PCR products of 344 bp for mouse S100A7/psoriasin and 330 bp for β-actin are shown. B Semi-quantitative analysis of the results shown in bottom panel of A Data points represent the means of three independent PCR reactions. The value of mouse S100A7 normalized to β-actin for each matched normal MG and MG tumor sample are joined by red lines. Blue lines represent the median value of mouse S100A7/psoriasin expression for each tissue type. Increased expression in MG tumors is significant (p < 0.05, one sided Wilcoxon signed rank test).

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