Table 3 Effects of in vitro transcribed ENO1 mRNA constructs on cell growth after transfection into neuroblastoma (SK-N-AS), 293 and K562 cell lines.
From: Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death
cell line | SK-N-AS | 293 | K562 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
mRNA | day | % decrease in number of cells +/-SD | no of exp | p-value test | % decrease in number of cells +/-SD | no of exp | p-value test | % decrease in number of cells +/-SD | no of exp | p-value test |
1.5 μg EGFP | - | 4 × 8 | - | 3 × 8 | - | 2 × 4 | ||||
1.5 μg ENO1 | 1 | 58 +/- 12 | 4 × 8 | < 10 -5 | 52 +/- 11 | 3 × 8 | < 10 -3 | -11 +/- 19 | 2 × 4 | > 0.05 |
2 | 81 +/- 11 | < 10 -9 | 85 +/- 8 | < 10 -5 | 13 +/- 34 | > 0.05 | ||||
3 | 79 +/- 12 | < 10 -9 | 89 +/- 16 | < 10 -5 | 9 +/- 11 | > 0.05 | ||||
4 | 76 +/- 17 | < 10 -9 | 87 +/- 20 | < 10 -5 | 10 +/- 9 | 0.03 | ||||
0.3 μg ENO1 | 1 | 39 +/- 25 | 3 × 8 | 0.02 | 38 +/- 27 | 2 × 8 | < 10 -4 | -14 +/- 14 | 1 × 4 | 0.04 |
2 | 63 +/- 15 | < 10 -5 | 64 +/- 36 | < 10 -4 | 6 +/- 11 | > 0.05 | ||||
3 | 60 +/- 11 | < 10 -5 | 60 +/- 54 | < 10 -4 | 4 +/- 10 | > 0.05 | ||||
4 | 64 +/- 13 | < 10 -3 | 59 +/- 52 | < 10 -5 | -3 +/- 9 | > 0.05 | ||||
1.5 μg ENO1-mut | 1 | 65 +/- 26 | 2 × 8 | 0.007 | 44 +/- 9 | 1 × 8 | < 10 -3 | -6 +/- 14 | 2 × 4 | > 0.05 |
2 | 84 +/- 8 | < 10 -3 | 77 +/- 4 | < 10 -6 | 20 +/- 8 | 0.004 | ||||
3 | 84 +/- 8 | < 10 -4 | 73 +/- 6 | < 10 -4 | 16 +/- 13 | 0.03 | ||||
4 | 84 +/- 7 | < 10 -3 | 63 +/- 6 | < 10 -6 | 5 +/- 11 | > 0.05 | ||||
0.3 μg ENO1-mut | 1 | 66 +/- 8 | 1 × 8 | < 10 -3 | 20 +/- 13 | 1 × 8 | 0.02 | -18 +/- 13 | 1 × 4 | 0.01 |
2 | 82 +/- 4 | < 10 -6 | 50 +/- 7 | < 10 -4 | 8 +/- 12 | > 0.05 | ||||
3 | 70 +/- 7 | < 10 -4 | 28 +/- 15 | 0.007 | 9 +/- 13 | > 0.05 | ||||
4 | 72 +/- 6 | < 10 -4 | 29 +/- 6 | < 10 -4 | -1 +/- 11 | > 0.05 | ||||
1.5 μg MBP-1 | 1 | 45 +/- 21 | 4 × 8 | < 10 -3 | 41 +/- 8 | 3 × 8 | 0.005 | -9 +/- 16 | 2 × 4 | > 0.05 |
2 | 67 +/- 13 | < 10 -7 | 75 +/- 10 | < 10 -4 | 10 +/- 12 | > 0.05 | ||||
3 | 68 +/- 15 | < 10 -6 | 82 +/- 25 | < 10 -4 | 1 +/- 8 | > 0.05 | ||||
4 | 64 +/- 20 | < 10 -7 | 76 +/- 31 | < 10 -4 | 5 +/- 9 | > 0.05 | ||||
0.3 μg MBP-1 | 1 | 25 +/- 18 | 3 × 8 | > 0.05 | 50 +/- 38 | 2 × 8 | 0.02 | -14 +/- 14 | 1 × 4 | 0.04 |
2 | 47 +/- 18 | 0.001 | 56 +/- 18 | < 10 -5 | 4 +/- 18 | > 0.05 | ||||
3 | 38 +/- 7 | 0.001 | 44 +/- 46 | < 10 -3 | -11 +/- 17 | > 0.05 | ||||
4 | 37 +/- 19 | 0.006 | 42 +/- 34 | < 10 -4 | -2 +/- 8 | > 0.05 | ||||