Cell growth curves of cDNA-transfected cells monitored for 3 days after transfection. The average number of cells in 8 separate experiments is shown at each time point for each construct. Standard deviation is indicated with a bar. A: Cell growth after transfection of five different constructs compared to empty vector (pIRES-EGFP) control in SK-N-AS cells. Using Students T-test, no significant differences in cell number compared to control cells could be detected for the first two days after transfection for any of the constructs, three days after transfection DFFA (p = 0.002), CORT (p = 0.004) and PGD (p < 0.001) showed significant difference. B: Cell growth after transfection of ENO1 compared to empty vector control. Significant differences in cell number was detected day 1 (p < 10-8) and day 2 (p < 10-4), the difference was not significant day 3 after transfection.