Mutations in APC , CTNNB1 and K-ras genes and expression of hMLH1 in sporadic colorectal carcinomas from the Netherlands Cohort Study

Table 1 Description of patient, tumour and molecular characteristics of colorectal cancers harbouring a CTNNB1 mutation.

Patient^a	Sex	Age at diagnosis (yr)	Sub-localisation	Dukes' stage	Tumour differentiation	Codon	Mutation	Amino acid change	hMLH1 expression^b	Truncating APC mutation^b	Activating K-ras mutation
1	M	65	Proximal colon	B	Moderate	37	C→T	Ser→Phe	Present	No	Yes
2	M	67	Proximal colon	A	Moderate	45	C→T	Ser→Phe	Absent	No	Yes
3	M	69	Proximal colon	B	Good	45	C→T	Ser→Phe	Absent	No	No
4	M	61	Proximal colon	C	Moderate	45	C→T	Ser→Phe	Present	No	Yes
5	F	69	Proximal colon	A	Undifferentiated	45	C→T	Ser→Phe	Absent	No	Yes
6^c	M	69	Distal colon	B	Moderate	22	G→A	Val→Ile	Present	No	Yes
7^c	F	65	Proximal colon	A	Moderate	29	C→A	Ser→Tyr	Present	No	No

^a Tumours of 464 sporadic colorectal cancer patients were analysed.
^b 58 Tumours were selected based on absence of hMLH1 expression, 411 tumours studied were selected based on absence of a truncating APC mutation.
^c Two patients (numbers 6 and 7) harboured a CTNNB1 mutation that would not lead to loss of a known phosphorylation site.

ISSN: 1471-2407