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Table 1 Bacterial strains, plasmids (ApR-ampicillin-resistant), cell lines and mice

From: Use of a recombinant Salmonella enterica serovar Typhimurium strain expressing C-Raf for protection against C-Raf induced lung adenoma in mice

Name

Relevant characteristics/sequence

Source or reference

Bacterial strains:

  

E. coli DH5α

F-, ø80dlacZΔM15, Δ(lacZYA-argF)U169 deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1

Takaba

Salmonella enterica serovar

  

Typhimurium aroA SL7207

hisG46, DEL407 [aroA544::Tn10 (Tcs)]

Stocker, B. A. D.

Salmonella enterica serovar

  

Typhimurium LB5000

rk-, mk+

Stocker, B. A. D.

Plasmids:

  

pUC13-c-raf-1

c-raf cDNA in pUC13

[12]

pcDNA3

pCMV, ApR, Neomycin, SV 40, ColE

Invitrogene

pcDNA-craf

craf cDNA in pcDNA3

Troppmair, J

pMOhly1

ApR, hlyR, hlyC, hlyAs (encoding the hemolysin secretion signal), hlyB, hlyD

[13]

pMOhly-Raf

ApR, hlyR, hlyC, raf-hlyAs (encoding a C-Raf-hemolysin fusion), hlyB, hlyD

this study

Cell lines:

  

EL-4

spontaneous murine lymphoma

ATCC(Rockville, MD, USA)

EL-4Raf

pcDNA-craf transfected EL-4 cells

this study

SF9

insect cell line

Gibco

SF9 Raf

craf transfected SF9 cells

[28]

Mice:

  

C57BL/6JolaHsd

wild-type (H-2b)

Harlan-Winkelmann, Borche, Germany

BxB23

[(C57BL/6 × DBA-2)F1] expressing an oncogenically activated NH2-terminal deletion mutant c-Raf-1-BxB under the control of human SP-C promoter

[15]

BxB11

[(C57BL/6 × DBA-2)F1] expressing an oncogenically activated NH2-terminal deletion mutant c-Raf-1-BxB under the control of human SP-C promoter

[15]