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Table 1 Bacterial strains, plasmids (ApR-ampicillin-resistant), cell lines and mice

From: Use of a recombinant Salmonella enterica serovar Typhimurium strain expressing C-Raf for protection against C-Raf induced lung adenoma in mice

Name Relevant characteristics/sequence Source or reference
Bacterial strains:   
E. coli DH5α F-, ø80dlacZΔM15, Δ(lacZYA-argF)U169 deoR, recA1, endA1, hsdR17(rk-, mk+), phoA, supE44, λ-, thi-1, gyrA96, relA1 Takaba
Salmonella enterica serovar   
Typhimurium aroA SL7207 hisG46, DEL407 [aroA544::Tn10 (Tcs)] Stocker, B. A. D.
Salmonella enterica serovar   
Typhimurium LB5000 rk-, mk+ Stocker, B. A. D.
Plasmids:   
pUC13-c-raf-1 c-raf cDNA in pUC13 [12]
pcDNA3 pCMV, ApR, Neomycin, SV 40, ColE Invitrogene
pcDNA-craf craf cDNA in pcDNA3 Troppmair, J
pMOhly1 ApR, hlyR, hlyC, hlyAs (encoding the hemolysin secretion signal), hlyB, hlyD [13]
pMOhly-Raf ApR, hlyR, hlyC, raf-hlyAs (encoding a C-Raf-hemolysin fusion), hlyB, hlyD this study
Cell lines:   
EL-4 spontaneous murine lymphoma ATCC(Rockville, MD, USA)
EL-4Raf pcDNA-craf transfected EL-4 cells this study
SF9 insect cell line Gibco
SF9 Raf craf transfected SF9 cells [28]
Mice:   
C57BL/6JolaHsd wild-type (H-2b) Harlan-Winkelmann, Borche, Germany
BxB23 [(C57BL/6 × DBA-2)F1] expressing an oncogenically activated NH2-terminal deletion mutant c-Raf-1-BxB under the control of human SP-C promoter [15]
BxB11 [(C57BL/6 × DBA-2)F1] expressing an oncogenically activated NH2-terminal deletion mutant c-Raf-1-BxB under the control of human SP-C promoter [15]