Figure 4

Activation of STAT1, STAT3 and ERK signalling in paired epithelial cell cultures. Paired cultures of epithelial cells, as listed in Table 2, were analyzed for the relative changes in STAT1, STAT2 and ERK signalling mediated by the treatments listed at the bottom. The level of phosphorylation of the signalling proteins was expressed relative to the level determined in the OSM-treated normal epithelial cells in the corresponding pair of cultures (defined as 100). A, C and E represent 20 pairs of the combination normal/normal and B, D and E, represent 23 pairs of the combination of normal/abnormal (metaplasia, dysplasia, carcinoma). G and H, indicate the fold-changes in the level of phosphorylated ERK and STAT3 in the matched of culture pairs of normal/normal (left side panel) and normal/abnormal epithelial cells. The values for the 4 carcinoma cases are shown by open circles.