Figure 1
Expression of RARβ2 and CTAG1 by Northern analysis. Northern analysis of RARβ2 (A) and CTAG1 (B) in RARβ2-transduced MDA-MB-435 cells compared to vector-control cells. Using total RNA used in the arrays, Northern blots were probed with 32P-dCTP labelled RARβ2 or CTAG1 cDNA. The RARβ2 probe consists of ~1.2 kb KpnI-BamH1 digest fragment of pSG RARβ2 ([75], from Pierre Chambon). The CTAG1 probe was generated from a 463 bp reverse transcriptase PCR product that encompasses the Agilent 60-mer. Blots were sequentially probed with a cDNA for RPLP0 (36B4) as a loading and transfer control. Quantitation of relative transcript levels was normalized to RPLP0 by phosphorimaging using exposure levels within the linear range of detection, avoiding saturation. The RARβ2 mRNA includes elements transcribed from the retroviral vector [8].