Figure 3
Impact of photoreactivation on UVB-induced apoptosis. Apoptosis in XPA cells was monitored at a 24 hour time point following exposure to 300 J/m2 UV and photoreactivation-induced repair. Apoptosis was examined by PI staining and FACS, gating the sub-G1 population as apoptotic. Apoptotic values were normalized to empty vector containing cells (amount of apoptosis set as 100%). Bar graphs show the relative amount of apoptosis for control cells, cells expressing CPD-PL alone (40% rescue), cells expressing 6-4PP-PL alone (70% rescue), or cell expressing both-PLs (75% rescue).