Figure 5From: Phenotypic and functional analysis of lymphocytes infiltrating osteolytic tumors: use as a possible therapeutic approach of osteosarcoma Proliferation profile of TIL isolated from rat osteosarcoma. These data are representative of three independent tumors. TIL isolated from rat osteosarcoma biopsies by enzymatic treatment were cultured at 5 × 105 TIL per well (6-multiwell plate) for 28 days in X-VIVO 15 supplemented with 900 UI/ml IL-2. At day 23 (arrow), 20 × 103 irradiated (75 grays) autologous osteosarcoma cells were added in each well. TIL were manually numbered using the trypan blue staining.Back to article page