Figure 5

Representative dot plots of BrdUrd content (log FITC fluorescence) vs. DNA content (propidium iodide fluorescence) of Jurkat cells at different post-labelling times. Jurkat tumour cells were labelled with BrdUrd for 30 min and then fixed after 8 h (A), 10 h (B), 12 h (C) and 16 h (D). A. Labelled cells have not yet entered the S^ld compartment. B. Labelled cells start entering the S^ld compartment. C. Considerable proportions of labelled cells are present both in the S^ld and in the S^lu compartment. D. Labelled cells are leaving the S^lu compartment. The optimal time for measurement of cell kinetics in Jurkat cells with the proposed method appears to be 12 h.