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Table 3 Comparison of blast proliferation by cell cycle analysis using PI labelling and PKH67 labelling (PKH fluorescence intensity (a.u.) at Day 0 / PKH fluorescence intensity (a.u.) at Day 4) in 7 AML patients stimulated or not by mixture of cytokines and incubated with a tetrapeptide AcSDKP (amide or not). Sample 1: PKH67 labelled cells cultured without the mixture of cytokines. Sample 2: PKH67 labelled cells cultured with the mixture of cytokines. Sample 3: cells with cytokines and AcSDKP-NH2 in 10-9 M. Sample 4: cells incubated with AcSDKP in 10-5 M to which cytokines were added after one day of culture. Sample 5: cells incubated with AcSDKP in 10-9 M in which cytokines were added after one day of culture. Sample 6: cells incubated with AcSDKP-NH2 in 10-9 M to which cytokines were added after one day of culture. Sample 7: cells without cytokines and AcSDKP-NH2 in 10-9 M.

From: Usefulness of PKH fluorescent labelling to study leukemic cell proliferation with various cytostatic drugs or acetyl tetrapeptide – AcSDKP

Patients Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7
Be        
PKH ratio 1.24 1.25 1.26 1.27 1.28 1.3 1.26
G0/1 82 88 78 83 80 81 86
S 9 8 10 8 9 7 8
G2+M 9 4 12 9 11 12 6
Dur        
PKH ratio 1.2 1.5 1.51 1.49 1.48 1.51 1.25
G0/1 87 78 82 81 81 81 90
S 6 11 8 9 8 8 6
G2+M 7 11 10 10 11 11 4
Ko        
PKH ratio 1.51 1.49 1.50 1 1.12 1.11 1
G0/1 90 87 90 94 97 96 96
S 7 6 7 1 1 2 1
G2+M 3 7 3 5 2 2 3
Patients Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7
Pa        
PKH ratio 1.18 2.01 2.1 1.8 1.75 1.75 1.13
G0/1 86 73 70 71 70 73 90
S 3 17 17 18 19 19 5
G2+M 11 10 13 11 11 8 5
Ph        
PKH ratio 1.18 1.5 1.5 1.31 1.32 1.33 1.11
G0/1 85 78 82 83 76 83 87
S 2 12 12 9 11 9 2
G2+M 13 10 6 8 13 8 11
Al        
PKH ratio 1 1.37 1.25 1.01 1.09 1.07 1
G0/1 95 83 89 93 94 91 92
S 1 8 5 1 1 1 1
G2+M 4 9 6 6 5 8 7
Av        
PKH ratio 1.1 1.6 2 1.1 1.09 1.02 1
G0/1 92 66 63 90 89 90 94
S 1 16 17 1 1 1 1
G2+M 7 18 20 9 10 9 5
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