Usefulness of PKH fluorescent labelling to study leukemic cell proliferation with various cytostatic drugs or acetyl tetrapeptide – AcSDKP

Table 3 Comparison of blast proliferation by cell cycle analysis using PI labelling and PKH67 labelling (PKH fluorescence intensity (a.u.) at Day 0 / PKH fluorescence intensity (a.u.) at Day 4) in 7 AML patients stimulated or not by mixture of cytokines and incubated with a tetrapeptide AcSDKP (amide or not). Sample 1: PKH67 labelled cells cultured without the mixture of cytokines. Sample 2: PKH67 labelled cells cultured with the mixture of cytokines. Sample 3: cells with cytokines and AcSDKP-NH2 in 10^-9 M. Sample 4: cells incubated with AcSDKP in 10^-5 M to which cytokines were added after one day of culture. Sample 5: cells incubated with AcSDKP in 10^-9 M in which cytokines were added after one day of culture. Sample 6: cells incubated with AcSDKP-NH2 in 10^-9 M to which cytokines were added after one day of culture. Sample 7: cells without cytokines and AcSDKP-NH2 in 10^-9 M.

Patients	Sample 1	Sample 2	Sample 3	Sample 4	Sample 5	Sample 6	Sample 7
Be
PKH ratio	1.24	1.25	1.26	1.27	1.28	1.3	1.26
G0/1	82	88	78	83	80	81	86
S	9	8	10	8	9	7	8
G2+M	9	4	12	9	11	12	6
*Dur*
PKH ratio	1.2	1.5	1.51	1.49	1.48	1.51	1.25
G0/1	87	78	82	81	81	81	90
S	6	11	8	9	8	8	6
G2+M	7	11	10	10	11	11	4
Ko
PKH ratio	1.51	1.49	1.50	1	1.12	1.11	1
G0/1	90	87	90	94	97	96	96
S	7	6	7	1	1	2	1
G2+M	3	7	3	5	2	2	3
Patients	Sample 1	Sample 2	Sample 3	Sample 4	Sample 5	Sample 6	Sample 7
Pa
PKH ratio	1.18	2.01	2.1	1.8	1.75	1.75	1.13
G0/1	86	73	70	71	70	73	90
S	3	17	17	18	19	19	5
G2+M	11	10	13	11	11	8	5
Ph
PKH ratio	1.18	1.5	1.5	1.31	1.32	1.33	1.11
G0/1	85	78	82	83	76	83	87
S	2	12	12	9	11	9	2
G2+M	13	10	6	8	13	8	11
Al
PKH ratio	1	1.37	1.25	1.01	1.09	1.07	1
G0/1	95	83	89	93	94	91	92
S	1	8	5	1	1	1	1
G2+M	4	9	6	6	5	8	7
Av
PKH ratio	1.1	1.6	2	1.1	1.09	1.02	1
G0/1	92	66	63	90	89	90	94
S	1	16	17	1	1	1	1
G2+M	7	18	20	9	10	9	5

ISSN: 1471-2407