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Table 2 Comparison between BrdUrd incorporation and PKH67 labelling in 17 AML patients treated with various drugs (DNR, AraC, VP16, Mitox, and AMSA). Percentage distribution of patient cells in each cell cycle phase obtained by BrdUrd incorporation and PKH67 labelling ratio at day 4 (PKH fluorescence intensity at Day 0 by PKH fluorescence intensity at Day 4).

From: Usefulness of PKH fluorescent labelling to study leukemic cell proliferation with various cytostatic drugs or acetyl tetrapeptide – AcSDKP

GI Control AraC DNR AraC + DNR  
G0/1 89 88 74 72  
S 8 6 8 4  
G2+M 3 6 18 24  
PKH ratio 1,4 1,35 1,37 1,25  
Lh Control AraC DNR AraC + DNR  
G0/1 57 53 54 53  
S 33 39 16 23  
G2+M 10 8 30 24  
PKH ratio 3,7 2,32 2,09 1,93  
Da Control AraC VP16 Mitox AraC + VP16 + Mitox
G0/1 74 76 66 73 71
S 7 10 10 12 17
G2+M 19 14 24 15 12
PKH ratio 1,6 1,4 1,4 1,5 1,4
Go Control AraC VP16 Mitox AraC + VP16 + Mitox
G0/1 60 64 85 71 75
S 16 10 4 8 2
G2+M 24 26 11 21 23
PKH ratio 1,6 1,3 1,6 1,6 1,3
Sat Control AraC VP16 Mitox AraC + VP16 + Mitox
G0/1 82 87 89 89 89
S 14 7 4 1 1
G2+M 4 6 7 10 10
PKH ratio 1,7 1,1 1,1 1,1 1,1
Le Control AraC VP16 Mitox AraC + VP16 + Mitox
G0/1 70 61 56 77 77
S 14 19 16 2 1
G2+M 16 20 28 21 22
PKH ratio 2,3 1,12 1,07 1,05 1,05
Fo Control AraC AMSA DNR DNR+AraC+AMSA
G0/1 81 75 90 71 90
S 14 20 2 6 2
G2+M 5 5 8 23 8
PKH ratio 1,7 1,1 1,18 1,18 1,18
Ko Control AraC DNR VP16 AraC+DNR+VP16
G0/1 71 80 70 74 76
S 13 2 8 4 3
G2/M 16 18 22 22 21
PKH ratio 1,5 1,05 1,13 1,04 1,06
Sa Control AraC DNR AraC+DNR  
G0/1 56 71 76 76  
S 32 10 9 5  
G2+M 12 19 17 19  
PKH ratio 3 1,4 1,1 1,12  
Na Control AraC DNR AraC+DNR  
G0/1 65 74 57 73  
S 31 21 1 23  
G2+M 4 5 42 4  
PKH ratio 3 2,5 1,13 1,11  
Pa Control AraC DNR AraC+DNR  
G0/1 89 90 87 89  
S 3 1 1 1  
G2+M 8 9 12 10  
PKH ratio 1,18 1,1 1,1 1,1  
La Control AraC DNR AraC+DNR  
G0/1 83 85 60 80  
S 11 7 3 13  
G2+M 6 8 37 7  
PKH ratio 1,02 1,01 1,1 1,1  
Luu Control AraC DNR AraC+DNR  
G0/1 86 82 86 85  
S 1 1 2 3  
G2+M 13 17 12 12  
PKH ratio 1,1 1,12 1,13 1,12  
Av Control AraC DNR AraC+DNR  
G0/1 68 65 80 68  
S 20 16 13 15  
G2+M 12 19 7 17  
PKH ratio 1,09 1,07 1,08 1,1  
Ph Control AraC DNR AraC+DNR  
G0/1 74 75 77 79  
S 1 2 3 1  
G2+M 25 23 20 20  
PKH ratio 1,1 1,03 1,05 1,07  
Li Control DNR    
G0/1 70 73    
S 11 7    
G2+M 19 20    
PKH ratio 1,07 1,08    
To Control DNR    
G0/1 92 73    
S 5 5    
G2+M 3 22    
PKH ratio 1,19 1,18    
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