Table 2 Comparison between BrdUrd incorporation and PKH67 labelling in 17 AML patients treated with various drugs (DNR, AraC, VP16, Mitox, and AMSA). Percentage distribution of patient cells in each cell cycle phase obtained by BrdUrd incorporation and PKH67 labelling ratio at day 4 (PKH fluorescence intensity at Day 0 by PKH fluorescence intensity at Day 4).
GI | Control | AraC | DNR | AraC + DNR | |
|---|---|---|---|---|---|
G0/1 | 89 | 88 | 74 | 72 | |
S | 8 | 6 | 8 | 4 | |
G2+M | 3 | 6 | 18 | 24 | |
PKH ratio | 1,4 | 1,35 | 1,37 | 1,25 | |
Lh | Control | AraC | DNR | AraC + DNR | |
G0/1 | 57 | 53 | 54 | 53 | |
S | 33 | 39 | 16 | 23 | |
G2+M | 10 | 8 | 30 | 24 | |
PKH ratio | 3,7 | 2,32 | 2,09 | 1,93 | |
Da | Control | AraC | VP16 | Mitox | AraC + VP16 + Mitox |
G0/1 | 74 | 76 | 66 | 73 | 71 |
S | 7 | 10 | 10 | 12 | 17 |
G2+M | 19 | 14 | 24 | 15 | 12 |
PKH ratio | 1,6 | 1,4 | 1,4 | 1,5 | 1,4 |
Go | Control | AraC | VP16 | Mitox | AraC + VP16 + Mitox |
G0/1 | 60 | 64 | 85 | 71 | 75 |
S | 16 | 10 | 4 | 8 | 2 |
G2+M | 24 | 26 | 11 | 21 | 23 |
PKH ratio | 1,6 | 1,3 | 1,6 | 1,6 | 1,3 |
Sat | Control | AraC | VP16 | Mitox | AraC + VP16 + Mitox |
G0/1 | 82 | 87 | 89 | 89 | 89 |
S | 14 | 7 | 4 | 1 | 1 |
G2+M | 4 | 6 | 7 | 10 | 10 |
PKH ratio | 1,7 | 1,1 | 1,1 | 1,1 | 1,1 |
Le | Control | AraC | VP16 | Mitox | AraC + VP16 + Mitox |
G0/1 | 70 | 61 | 56 | 77 | 77 |
S | 14 | 19 | 16 | 2 | 1 |
G2+M | 16 | 20 | 28 | 21 | 22 |
PKH ratio | 2,3 | 1,12 | 1,07 | 1,05 | 1,05 |
Fo | Control | AraC | AMSA | DNR | DNR+AraC+AMSA |
G0/1 | 81 | 75 | 90 | 71 | 90 |
S | 14 | 20 | 2 | 6 | 2 |
G2+M | 5 | 5 | 8 | 23 | 8 |
PKH ratio | 1,7 | 1,1 | 1,18 | 1,18 | 1,18 |
Ko | Control | AraC | DNR | VP16 | AraC+DNR+VP16 |
G0/1 | 71 | 80 | 70 | 74 | 76 |
S | 13 | 2 | 8 | 4 | 3 |
G2/M | 16 | 18 | 22 | 22 | 21 |
PKH ratio | 1,5 | 1,05 | 1,13 | 1,04 | 1,06 |
Sa | Control | AraC | DNR | AraC+DNR | |
G0/1 | 56 | 71 | 76 | 76 | |
S | 32 | 10 | 9 | 5 | |
G2+M | 12 | 19 | 17 | 19 | |
PKH ratio | 3 | 1,4 | 1,1 | 1,12 | |
Na | Control | AraC | DNR | AraC+DNR | |
G0/1 | 65 | 74 | 57 | 73 | |
S | 31 | 21 | 1 | 23 | |
G2+M | 4 | 5 | 42 | 4 | |
PKH ratio | 3 | 2,5 | 1,13 | 1,11 | |
Pa | Control | AraC | DNR | AraC+DNR | |
G0/1 | 89 | 90 | 87 | 89 | |
S | 3 | 1 | 1 | 1 | |
G2+M | 8 | 9 | 12 | 10 | |
PKH ratio | 1,18 | 1,1 | 1,1 | 1,1 | |
La | Control | AraC | DNR | AraC+DNR | |
G0/1 | 83 | 85 | 60 | 80 | |
S | 11 | 7 | 3 | 13 | |
G2+M | 6 | 8 | 37 | 7 | |
PKH ratio | 1,02 | 1,01 | 1,1 | 1,1 | |
Luu | Control | AraC | DNR | AraC+DNR | |
G0/1 | 86 | 82 | 86 | 85 | |
S | 1 | 1 | 2 | 3 | |
G2+M | 13 | 17 | 12 | 12 | |
PKH ratio | 1,1 | 1,12 | 1,13 | 1,12 | |
Av | Control | AraC | DNR | AraC+DNR | |
G0/1 | 68 | 65 | 80 | 68 | |
S | 20 | 16 | 13 | 15 | |
G2+M | 12 | 19 | 7 | 17 | |
PKH ratio | 1,09 | 1,07 | 1,08 | 1,1 | |
Ph | Control | AraC | DNR | AraC+DNR | |
G0/1 | 74 | 75 | 77 | 79 | |
S | 1 | 2 | 3 | 1 | |
G2+M | 25 | 23 | 20 | 20 | |
PKH ratio | 1,1 | 1,03 | 1,05 | 1,07 | |
Li | Control | DNR | |||
G0/1 | 70 | 73 | |||
S | 11 | 7 | |||
G2+M | 19 | 20 | |||
PKH ratio | 1,07 | 1,08 | |||
To | Control | DNR | |||
G0/1 | 92 | 73 | |||
S | 5 | 5 | |||
G2+M | 3 | 22 | |||
PKH ratio | 1,19 | 1,18 |