Figure 2
From: Anthracyclines, proteasome activity and multi-drug-resistance
Anthracyclines are inhibitors of proteasome function. Incubation of ECV304 cells stably transfected with an Ub-GFP fusion protein with daunorubicin (100 μM, 16 h), caused accumulation of GFP throughout the cytoplasm (lower picture), indicating proteasome inhibition in living cells while untreated controls cells showed only little accumulation of GFP (A/B). Daunorubicin accumulated in the perinuclear region (C).