(A) Real-time RT-PCR analysis of RNA from breast tumours. Each block on the × axis represents a different tumour. The amount of eEF1A2 message is shown normalised to GAPDH and expressed relative to the level of expression in the normal breast RNA samples (=1). ER-negative tumours are shown in white, ER-positive tumours are shown in black. The difference in mean expression between ER-positive and ER-negative samples is 7.2 units (p = 0.0087), 95% Confidence Interval 2.0 to 12.4 units. (B) Average standardised RNA levels in ER-negative and ER-positive breast tumours. This difference is significant (P = 0.0087, t-test).