Lack of GIRK1 expression in fresh surgical tissue samples of pulmonary adenocarcinomas or pulmonary squamous carcinomas. Top panel: GIRK1 was not expressed in two pulmonary squamous carcinomas or three adenocarcinomas obtained from cancer patients. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; cyclophilin-216 bp; M-100 bp.