Expression of GIRK channels in adenocarcinoma alveolar type II cell phenotype (A549) and carcinoid (H727) cell lines. Top panel: GIRK1 was expressed in both A549 and H727 cell lines. Bottom panel: Both A549 and H727 expressed GIRK2 and GIRK4. Cyclophilin, used as a positive reaction control was seen in both samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; GIRK2-438 bp; GIRK4-401 bp; cyclophilin-216 bp; M-100 bp.