Figure 6
Effect of psammaplin A on pol α-primase activity. Indicated amounts of psammaplin A were added to the reaction mixtures, which included 0.1 unit of human pol α-primase complex, 4 mM ATP, 33 μM of [3H]dTTP, 0.1 μg of poly (dA)4500:oligo(dT)25(20:1), incubations were performed at 37°C for 30 min, followed by measurement of acid-insoluble radioactivity. Data are shown as means ± SD of three independent experiments. * Data are significantly different from control group at p < 0.001.