Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: Opposite role of Bax and BCL-2 in the anti-tumoral responses of the immune system

Figure 1

A. Western blot analyses of human Bcl-2 and Bax transgenes expression in stable A15A5 transfectants. Two clones per transfection with pCMV (E12 and C4), pCMV-Bcl-2 (Bcl-2: D6 and D4), pCMV-Bax (Bax: H11 and E4), which expressed approximately the same amount of trangenes were selected to be used in this study. B. The effect of huBcl-2 and huBax on the sensitivity of A15A5 cells to apoptosis, transfected cells was analysed after incubated with various cell death inducers as described in materials and methods. Apoptosis was determined by measuring the specific DEVDase and LDH activities after treatment of the cells with doxorubicin (doxo), staurosporine (STS), Na-Butyrate (NaB), serum deprivation (d-serum) or UV irradiation (UV). S.D. was calculated from 3 different experiments using 2 independent clones for each transgene. C. Cell death was induced by FasL and GrB. A15A5 cells were treated with FasL (0.5 μg/ml) or were transfected with a plasmid encoding for human GrB (200 μg plasmid) as described in 21. In the latter case, we verified by FACS that similar amounts of human GrB were expressed in all transfected clones (data not shown). S.D. was calculated from 3 different experiments using 2 independent clones for each transgene.

Back to article page