Figure 5

DNA fragmentation, PARP and Lamin B cleavage in rS100A4/IFN-γ-treated II-11b cells. A, Ethidium bromide stained agarose gel of DNA isolated from II-11b cells. Cells were stimulated or left untreated with 100 u/ml IFN-γ for 72 hours in the presence or absence of 20 μg/ml rS100A4 as indicated. B, Western blot analysis of the expression of PARP and Lamin B in total cell lysates. II-11b cells were left untreated or treated with 100 u/ml IFN-γ and/or 20 μg/ml rS100A4 for 24, 48 and 72 hours as indicated, and lysates subjected to Western blot analysis as described in "Materials and Methods".