Figure 3From: Sensitization of interferon-γ induced apoptosis in human osteosarcoma cells by extracellular S100A4 OHS cells secrete S100A4. Immunoprecipitation of cell culture medium alone (control) or cell culture medium from II-11b and OHS cells cultured for 24, 48 and 72 hours as indicated. 1.5 ml of medium was immunoprecipitated using rabbit polyclonal anti-S100A4 or anti-p300, and subsequently immunoblotted with anti-S100A4.Back to article page