Table 2 Oligonucleode primer sequences.
Gene | 5' sequence | 3' sequence | Standard amplicon | Competitor sequence (standard 5' primer plus) | Competitor amplicon |
|---|---|---|---|---|---|
MT1-MMP | gACAgTACACCCTTTgATggTgAA | gATCgTTAgAATgTTCCAggCCTA | 197 bp | CAgGCCCCAATATTggAggggAT | 169 (-28 bp) |
MMP-11 | CCTTCCAggATgCTgAgggCTAT | ATgACAgCATggTCgTTCCTACAA | 330 bp | TCTCTACTggAggTTTgATCCCgT | 310 (-20 bp) |
18s rRNA | TCAAGAACgAAAgTCggAgg | ggACATCTAAgggCATCACA | 490 bp | N/A | N/A |