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Table 1 Secreted cytokine array. Secreted proinflammatory cytokines down-regulated by HA, anti-MIF antibody and MIF anti-sense. Integrated density values (IDV, average intensity times total area) were determined for each individual cytokine dot. Individual cytokine dot intensities were normalized to HRP-positive controls on each membrane. Mean control data for each cytokine spot are expressed as protein ratios (cytokine specific band integrated density value divided by HRP-positive control integrated density value). Treatment data are expressed as +, greater than 2 fold increase in secretion compared with control cytokine spot; -, greater than 2 fold decrease in secretion compared with control cytokine spot; (-), decreased beyond detection.

From: Inhibition of macrophage migration inhibitory factor decreases proliferation and cytokine expression in bladder cancer cells

Cytokine Family      
   Control (Mean Protein Ratio) HA α-MIF AS
Chemokine CC      
  CCL2 1.27 ± 0.04 - (-)  
  CCL4 0.30 ± 0.02 - (-) (-)
  CCL5 0.18 ± 0.01 - (-) (-)
  CCL11 0.11 ± 0.01 (-) (-) (-)
  CCL15 0.41 ± 0.04 (-) (-) -
  CCL17 0.12 ± 0.01 - (-) (-)
  CCL18 1.97 ± 0.07 - (-) -
  CCL20 0.69 ± 0.04 (-)   -
  CCL22 0.19 ± 0.02 - (-) -
  CCL23 0.12 ± 0.01 (-) (-) (-)
  CCL24 0.14 ± 0.01 - (-) -
  CCL26 0.20 ± 0.01 (-) (-) (-)
CXC Chemokine      
  CXCL1 0.16 ± 0.02 - - (-)
  CXCL2 0.15 ± 0.02 - - (-)
  CXCL5 0.19 ± 0.02 - (-) (-)
  CXCL6 0.26 ± 0.03 - - -
  CXCL7 1.56 ± 0.03 - (-) -
  CXCL8 0.32 ± 0.02 -   -
  CXCL9 0.16 ± 0.01 - (-) -
  CXCl10 1.60 ± 0.17 - - -
  CXCL12 0.15 ± 0.01 - (-) -
  CXCL13 0.34 ± 0.02 - (-) -
CXC3C Chemokine      
  CX3CL1 0.19 ± 0.01 -   (-)
Cytokines      
  Leptin 0.19 ± 0.02 - (-) (-)
  LIF 3.20 ± 0.04 -   
  MIF 0.64 ± 0.04 (-) (-) (-)
  OSM 0.20 ± 0.02 - (-) (-)
Interferon      
  INF-γ 0.17 ± 0.02 - (-) (-)
Neurotropins      
  BDNF 1.10 ± 0.05 -   -
  GDNF 1.30 ± 0.07 -   
  NT-3 2.00 ± 0.11 - (-)  
  NT-4 0.79 ± 0.04 - (-) -
Enzymes TIMP-1 2.54 ± 0.11 -   
  TIMP-2 5.29 ± 0.29 - +  
Growth Factors      
  Ang-1 0.20 ± 0.02 (-)   (-)
  EGF 0.17 ± 0.03 - (-) -
  FGF-4 0.24 ± 0.03 - (-) (-)
  FGF-6 0.18 ± 0.01 - (-) -
  FGF-9 0.81 ± 0.05 -   
  HGF 0.12 ± 0.02 - (-) (-)
  MCSF 0.15 ± 0.02 - (-) (-)
  PGIF 0.64 ± 0.05 - (-) -
  Inhibin 0.17 ± 0.02 - (-) (-)
  TGF β-2 3.20 ± 0.03 -   
  TGF β-3 0.77 ± 0.02 - (-) (-)
Hormone-like protein      
  IGF-1 0.14 ± 0.01 (-) (-) (-)
  IGFBP-1 2.40 ± 0.10 -   
  IGFBP-2 1.40 ± 0.06 -   -
  IGFBP-3 2.50 ± 0.12 - (-)  
  IGFBP-4 0.11 ± 0.01 - (-) -
  TP-3 0.13 ± 0.01 - (-) (-)
Interleukins      
  IL-1α 0.20 ± 0.01 - (-) (-)
  IL-1β 0.14 ± 0.02 - (-) (-)
  IL-2 0.14 ± 0.04 - (-) (-)
  IL-12 0.16 ± 0.01 - (-) (-)
  IL-15 0.10 ± 0.09 - (-) (-)
  IL-16 0.27 ± 0.04 - (-) -
PDGF Superfamily      
  PDGF-B 0.17 ± 0.02 - (-) (-)
  P1GF 0.59 ± 0.04 - (-) -
  VEGF 3.20 ± 0.48 - + -
TNF Superfamily      
  TNFSF14 0.19 ± 0.01 - - (-)
  TNFSF2 0.13 ± 0.02 (-) (-) -
  TNFRSF11B 4.40 ± 0.04 -   
CD Ligands      
  Fit-3 0.07 ± 0.01 - - (-)