Effect of MIF Inhibition on TNF-α and IL-1β gene expression – A. TNF-α expression – TNF-α mRNA content was quantified in cells following 24 h with the indicated treatment. Anti-MIF and MIF antisense treatments decreased the levels of TNF-alpha mRNA. HA treatment, however, was not effective. B. IL-1β expression – IL-1β mRNA content was quantified in cells following 24 h with the indicated treatment. No significant difference in IL-1β expression was seen with any of the treatments. In both A and B the data is expressed as a relative intensity ratio. PCR band intensity was determined from the formula, total intensity = area × average intensity. The relative intensity ratio is determined from the total intensity of gene specific PCR product band divided by the 18 S rRNA band intensity (internal standard), (* – p < 0.05, ** – p < 0.01).