Figure 6

Induction of apoptosis by IFNγ and MIBG A: IFNγ induced apoptosis specific caspase-3 activity in BON and STC-1 cells after 48 h of incubation. B: MIBG-treatment (48 h) dose-dependently increased caspase-3 activity of BON cells. Interestingly, combination treatment with IFNγ and MIBG resulted in sub-additive caspase-3 activation. Black bars indicate the calculated additive values of caspase-3 activation of either combination. C: MIBG-treatment (48 h) dose-dependently increased caspase-3 activity of STC-1 cells. Co-incubation with IFNγ and MIBG led to sub-additive caspase-3 activation. Black bars indicate the calculated additive values of caspase-3. Data are given as means ± SEM of 3 (STC-1) or 4 (BON) independent experiments. *, statistical significance (p < 0.05) compared with untreated controls.