mRNA expression of human norepinephrine transporters (hNET) mRNA expression of norepinephrine transporters was evaluated in BON cells. A: Transcripts specific for hNET were detected by RT-PCR (lane 1). β-actin was used as loading control (lane 2). PCR products were visualized by gel electrophoresis on an ethidium bromide-stained agarose gel. M: 100 bp DNA ladder. B: Up-regulation of hNET expression by IFNγ. Semi-quantitative RT-PCR revealed a dose-dependent induction of hNET expression in BON cells by IFNγ after 48 h. Data are given as means ± SEM of 2 experiments.