Scatter plot images and corresponding Pearson Coefficients between direct and indirect labeling methods. (A) Scatter plot of direct labeling replicates yielding a Pearson Coefficient of 0.948 for all genes (n = 7,476 genes). (B) Scatter plot of indirect labeling replicates yielding a Pearson Coefficient of 0.924 for all genes (n = 7,474). (C) A representative image of a comparison of the gene expression ratios between a direct and indirectly labeled slide. The average Pearson Coefficient generated comparing labeling methods was 0.895 for all genes (range 0.883–0.911, n = 7,474 genes). Extraction method for all genes is found in the methods.