Table 1 RT-PCR primer pairs used for mesothelin transcript amplification
From: Characterization of human mesothelin transcripts in ovarian and pancreatic cancer
amplification target | sequence, 5' – 3' | location | expected product size |
|---|---|---|---|
full length (tr1 & 2) | ACCCACGGTGCCTCCCTCCC TCAGGCCAGGGTGGAGGCTAG | exon 1 exon 17 | 1906 bp (tr1) 1924 bp (tr 2) |
24 bp insert region (tr1 & 2) | CTACCTCTTCCTCAAGATGAG GTGTCTAGGGTGTCTTTGTCT | exon 12 exon 13 | 177 bp (tr1) 201 bp (tr2) |
82 bp insert region (tr1 & 3) | ATGAAGCTGCGGACGGATGCG TGAGAACAGGTCCAGGTCCTA | exon 15 exon 17 | 262 bp (tr 1) 344 bp (tr 3) |
tr3-specific | ACCCACGGTGCCTCCCTCCC ACCTCCACGCCCCCAGCTCTG | exon 1 intron 16 | 1873 bp |