Figure 3

RT-PCR analysis of mesothelin transcripts. Primers designed to amplify full length mesothelin from transcript variants 1 and 2 (Table 1, line 1) were used in RT-PCR analyses of total RNA from cell lines and tissues, and analyzed by agarose gel electrophoresis. M, molecular size markers; Lane 1, HeLa; Lane 2, OVCAR-3; Lane 3, Caov-4; Lane 4, SKOV-3; Lane 5, UCI 101; Lane 6, AsPC-1; Lane7, PANC-1; Lanes 8–10, three individual normal ovary tissues; Lanes 11–16, six individual primary ovarian tumors; Lane 17, no template PCR control.