|
Cells
|
5αR
|
20α-HSO (reductive)
|
3α-HSO (reductive)
|
5αR/20α-HSO
|
5αR/3α-HSO
|
|---|
|
MCF-7
|
228.6 (86.5)
|
17.9 (6.8)
|
17.9 (6.8)
|
12.77
|
12.77
|
|
MDA231
|
186.2 (75.3)
|
49.8 (20.1)
|
11.3 (4.6)
|
3.74
|
16.48
|
|
T-47D
|
82.1 (74.5)
|
21.8 (19.8)
|
6.3 (5.7)
|
3.77
|
13.03
|
|
MCF-10A
|
78.3 (7.6)
|
845.1 (81.4)
|
114.2 (11.0)
|
0.93
|
0.69
|
- The 10,000 × g supernatant of cell homogenates was incubated with NADH, NADPH and [3H]progesterone, and enzyme activities were determined by quantifying metabolites formed as a result of specific enzyme actions (See Methods for details). Values (mean from two experiments) are given as pg/hour/mg protein; the values in brackets show the percent of total enzyme activities for a particular cell type.
