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Table 2 5α-Reductase, 20α-HSO and 3α-HSO activities in microsomes/cytosol of breast cell lines.

From: Activity and expression of progesterone metabolizing 5α-reductase, 20α-hydroxysteroid oxidoreductase and 3α(β)-hydroxysteroid oxidoreductases in tumorigenic (MCF-7, MDA-MB-231, T-47D) and nontumorigenic (MCF-10A) human breast cancer cells

Cells 5αR 20α-HSO (reductive) 3α-HSO (reductive) 5αR/20α-HSO 5αR/3α-HSO
MCF-7 228.6 (86.5) 17.9 (6.8) 17.9 (6.8) 12.77 12.77
MDA231 186.2 (75.3) 49.8 (20.1) 11.3 (4.6) 3.74 16.48
T-47D 82.1 (74.5) 21.8 (19.8) 6.3 (5.7) 3.77 13.03
MCF-10A 78.3 (7.6) 845.1 (81.4) 114.2 (11.0) 0.93 0.69
  1. The 10,000 × g supernatant of cell homogenates was incubated with NADH, NADPH and [3H]progesterone, and enzyme activities were determined by quantifying metabolites formed as a result of specific enzyme actions (See Methods for details). Values (mean from two experiments) are given as pg/hour/mg protein; the values in brackets show the percent of total enzyme activities for a particular cell type.