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Table 1 Primer sequences (upstream; downstream) used in the RT-PCR analyses.

From: Activity and expression of progesterone metabolizing 5α-reductase, 20α-hydroxysteroid oxidoreductase and 3α(β)-hydroxysteroid oxidoreductases in tumorigenic (MCF-7, MDA-MB-231, T-47D) and nontumorigenic (MCF-10A) human breast cancer cells

cDNA Sequence Size Reference
SRD5A1 (5αR1) 5'-TGG CGC TTC TCT ATG GAC TT-3' 5'-GGA AGC AAC ACT GCA GTT GA-3' 368 bp [15]
SRD5A2 (5αR2) 5'-CAT ACG GTT TAG CTT GGG TGT-3' 5'-GCT TTC CGA GAT TTG GGG TAG-3' 315 bp [16]
AKR1C1 (20α(3α)HSD) 5'-GTA AAG CTT TAG AGG CCA C-3' 5'-CAC CCA TGC TTC TTC TCG G-3' 590 bp [43]
AKR1C2 (3αHSD3) 5'-GTA AAG CTC TAG AGG CCG T-3' 5'-CAC CCA TGG TTC TTC TCG A-3' 590 bp [43]
AKR1C3 (3α(17β)HSD2) 5'-GTA AAG CTT TGG AGG TCA C-3' 5'-CAC CCA TCG TTT GTC TCG T-3' 590 bp [43]
AKR1C4 (3αHSD1) 5'-ACA GAG CTG TAG AGG TCA C-3' 5-CAC CCA TAG TTT ATG TCG T-3' 590 bp [43]
HSD3B1 &HSD3B2 (3βHSD) 5'-GCT GGA GCT GCC TTG TGA C-3' 5'-CCC GGC TAC CTC TAT GCT ACT-3' 383 bp (M.J. Lewis)
18S rRNA 5'-GTA ACC CGT TGA ACC CCA TT-3' 5'-CCA TCC AAT CGG TAG TAG CG-3' 131 bp [17]