Table 1 Primer sequences (upstream; downstream) used in the RT-PCR analyses.
cDNA | Sequence | Size | Reference |
|---|---|---|---|
SRD5A1 (5αR1) | 5'-TGG CGC TTC TCT ATG GAC TT-3' 5'-GGA AGC AAC ACT GCA GTT GA-3' | 368 bp | [15] |
SRD5A2 (5αR2) | 5'-CAT ACG GTT TAG CTT GGG TGT-3' 5'-GCT TTC CGA GAT TTG GGG TAG-3' | 315 bp | [16] |
AKR1C1 (20α(3α)HSD) | 5'-GTA AAG CTT TAG AGG CCA C-3' 5'-CAC CCA TGC TTC TTC TCG G-3' | 590 bp | [43] |
AKR1C2 (3αHSD3) | 5'-GTA AAG CTC TAG AGG CCG T-3' 5'-CAC CCA TGG TTC TTC TCG A-3' | 590 bp | [43] |
AKR1C3 (3α(17β)HSD2) | 5'-GTA AAG CTT TGG AGG TCA C-3' 5'-CAC CCA TCG TTT GTC TCG T-3' | 590 bp | [43] |
AKR1C4 (3αHSD1) | 5'-ACA GAG CTG TAG AGG TCA C-3' 5-CAC CCA TAG TTT ATG TCG T-3' | 590 bp | [43] |
HSD3B1 &HSD3B2 (3βHSD) | 5'-GCT GGA GCT GCC TTG TGA C-3' 5'-CCC GGC TAC CTC TAT GCT ACT-3' | 383 bp | (M.J. Lewis) |
18S rRNA | 5'-GTA ACC CGT TGA ACC CCA TT-3' 5'-CCA TCC AAT CGG TAG TAG CG-3' | 131 bp | [17] |