Figure 6

TSA modulates expression of cell surface molecules. (A) PBMCs from C57BL/6 mice were cultured in the presence of soluble anti-CD3 antibody (0.5 μg/ml) or IL-2 (10 U/ml) and exposed to 100 nM TSA for 4 hours (open black histogram) and 20 hours (open blue histogram), or to DMSO alone for 4 hours (open red histogram) or 20 hours (open brown histogram). Cells were harvested, extensively washed, and flow cytometric analysis of cell surface molecules performed as described in the materials and methods section. Dead cells are excluded from the analysis. (B) PBMCs from C57BL/6 mice were cultured in the presence of soluble anti-CD3 antibody (0.5 μg/ml) or IL-2 (10 U/ml) and exposed to 100 nM TSA or to DMSO alone for 20 hours. Cells were harvested, stained with a PE-conjugated anti-CD4 antibody and analysed by flow cytometry. PI staining of nuclei assessed cell viability. Shown are the mean percentage of survival; bars, SD. The data are representative of three independent experiments.