Figure 4
From: Phenotypic alterations in breast cancer cells overexpressing the nuclear receptor co-activator AIB1
AIB1 transfected 436.1 cells display increased reporter gene activity. Cells weretransiently transfected with increasing amounts of estrogen receptor expression plasmidand 1.5 ug of pGL3. ERE. luc reporter vector and incubated in the presence or absence of ethanol (EtOH), 10 nM 17β-estradiol (E2), or 100 nM 4-hydroxytamoxifen (Tam). Results are representative of three experiments. Relative luciferase activity = Luciferase/Renilla.